5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Data-enriched characterization of cell populations. Morphological data from images can add on the richness of flow cytometry info. For example, the determine reveals an if not conventional apoptosis assay utilizing Annexin V and PI, incorporating mobile imaging to characterize cells in Every population to expose morphologically distinctive functions. These insights couldn't are actually gained from multiplex staining by itself.
Whether you’re a newbie or a specialist, check out our methods to progress your exploration and experience in flow cytometry.
(B) RAW 264.7 macrophages tend to be annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The quantity of singlets readily available for Evaluation raises after the consumer trains and employs a more accurate product.
Discover new insights into your experimental data by visualizing populations applying put together fluorescence and morphology details. Our software program interface wizards permit downsampling, concatenation (merged data files) and dimensionality resources which might be built in to the application for an intuitive, streamlined data Investigation experience.
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Below, a highly skilled person has gated singlets confidently. Just after assessing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate has in excess of four% aggregates.
Pair your Attune flow cytometer with an autosampler for enhanced workflow effectiveness, and insert robotic integration for optimum throughput.
Reliable graphic good quality even at superior throughput Acoustic focusing in addition to a significant-velocity camera combine to impression these T cells constantly at minimal or substantial flow charges. Very easily change concentration and camera configurations to satisfy experimental specifications.
Early signs of this contamination experienced to begin with been dismissed as particles. Backgating to discover new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also means that you can use morphological here features to discover appealing subpopulations that may not be clear from flow cytometry data on your own.
Buyers can see event pictures in true-time to confirm sample quality, optimize protocols, and record the morphology of every cell populace discovered for analysis. Imaging capture also benefits from acoustic concentrating, which helps you to placement the cells to ensure that a pointy, centered graphic is obtained.
AI-pushed automated impression Evaluation While using the Attune Cytometric Software package interprets celebration capabilities into distinct morphology parameters that may be coupled with standard fluorescence and scatter parameters to expose very reproducible inhabitants alterations and distinctive subsets.
Application note: Automatic graphic analysis lowers user-to-consumer variability in flow cytometry gating techniques
Designed-in dimensionality reduction applications improved resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm predicted population resolution in lysed full human blood. User can check out events from each cluster for Visible affirmation of mobile phenotypes.
No wash/no lyse leukocyte analysis with imaging. Cells have been obtained from 24-hour-aged blood by dilution in one mM EDTA ( Skilled UX/UI allows users to prepare AI models for expanded programs. (A) The “Prepare Design” feature offers annotation instruments and actual-time model training precision.
At the center of each Attune Flow Cytometer lies our revolutionary acoustic concentrating know-how, which utilizes audio waves to meticulously align cells within the flow cell. This modern solution surpasses standard hydrodynamic approaches, causing quicker processing speeds, Fantastic facts high quality, and enhanced sample throughput.
The Attune Xenith cytometer is a superior-parameter spectral analyzer, offering each spectral unmixing and conventional flow abilities for extensive and flexible mobile Examination.
The Attune CytPix Flow Cytometer allows buyers to visualize samples in the course of acquisition, validate the precision of gating procedures, and assist outcomes with reliable documentation for publication needs.
Listed here, we exhibit that by gating tactics employing picture-primarily based quantitative parameters can distinguish interacting cells from coincident events to more accurately evaluate interacting cells.